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“We found geographical and chronological evidence suggesting that the major vicariant events between D. antonietae and the D. serido haplogroup, as well as the demographic fluctuations in the lineages in the latter group, could be related to the causal effects of Pleistocene climatic changes on the spatial dynamics of the Seasonally Dry Tropical Forests in Brazil.” “The phylogeographical and genetic patterns observed for D. gouveai in this study are consistent with changes in the distribution of dry vegetation in eastern Brazil. All of the estimates obtained by molecular dating indicate that range expansion and isolation pre-dated the Last Glacial Maximum, occurring during the late to middle Pleistocene, and were probably triggered by climatic changes during the Pleistocene.” “Here we describe the isolation and evaluation of 11 microsatellite loci for P. machrisii and test the transferability of those markers to other related species belonging to Pilosocereus.” “…the cross-amplified microsatellite markers were able to reveal differing results between the genetic variation and taxonomic separation involving both P. machrisii and P. aurisetus populations.” “…events of introgression were highlighted to refute the idea of period as a speciation gene in these groups. Due the introgression evidences found in the present paper, this same suggestion could be also ascribed to the period gene fragment of the D. buzzatii cluster species.”
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